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Factors such as high pressure and speed, during sorting, dye-induced defects, electrical deviation, changes in pH and osmolarity, etc might lead to changes to the sperm plasma membrane, trigger pre capacitation-like changes, cause protamine decondensation and alter sperm DNA quality (68)

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MK-677 (half-life 24 hours) requires a longer taper 3-4 weeks minimum because its prolonged receptor occupancy causes deeper suppression that takes 28-35 days to fully resolve