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Then the sample was injected onto the trap column with a flow rate of 10 L/min and subsequently eluted with a five-step linear gradient (A: ddH 2 O with 0.1% formic acid, B: ACN with 0.1% formic acid): 010 min, 38% B

Briefly, HT-1080 cells were seeded into 15-cm dishes at a density of 1 10 6 cells/dish, then the next day treated nutlin-3 (10 M)

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