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Cell viability was assessed 3 days after treatment with FOXO4-DRI at 0, 12.5, 25, 50, and 100 M, respectively, using the Cell Counting Kit-8 (CCK-8) Assay (Dojindo, Rockville, MD, United States)

Despite increased glycolysis attempting to offset reduced FAO, this approach yields approximately 30% less ATP than a healthy heart does, which is insufficient to meet energy needs