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Dogs with the ACE variant had lower levels of ACE activity even in more advanced mitral valve disease than dogs without the variant." They concluded that the impact of their finding "on the need for ACE-I in dogs with the polymorphism and heart disease deserves further study." Then, in a September 2023 abstract, the same authors' aim was to determine the impact of certain angiotensin converting enzyme (ACE) gene polymorphisms upon the presence of congestive heart failure (CHF) in MVD-affected cavaliers

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Garcia in "The NT-proBNP assay: A portent of heart health." in dvm360: "For conditions such as mitral valve disease, this test may be of limited value because a diagnosis can be readily made by thorough auscultation and documentation of a heart murmur