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therefore, we mainly focused on loops 3 and 5 to investigate if their sequence variations are linked to their distinctive substrate differences

Research has demonstrated that excessive ROS can ultimately result in both single-stranded and double-stranded DNA damage, impairing cell proliferation and intercellular adhesion, which may contribute to genomic instability and cell death (Sehitogullari et al., 2014)

Statistical analysis A combination of multivariate (orthogonal partial least squares discriminant analysis) and univariate (analysis of variance, linear modeling) approaches was applied to perform group comparisons, following the workflow described by Blaise et al