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Three technical replicates were performed for each sample and the mean cycle threshold (Ct) was calculated

They also calculated that they would need to review 158 specific recommendations to see whether their hypothesis was correct

10.7150/thno.62330 385 XuH.LiH.ZhangP.GaoY.MaH.GaoT.et al (2024)

pH stability profiles Appropriate dilutions of purified laccase variants were prepared such that 20-l aliquots of enzymes in reaction mix gave rise to a linear response in kinetic mode