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On the next day, cells were transfected with SOD1 WT (1 ug) or SOD1 G93A (1 ug) using transfection reagent Lipofectamine 2000 (ThermoFisher Scientific, Waltham, MA)

The key insight: The medication is a tool

The kynurenine pathway (KP), which catabolizes over 95% of tryptophan via indoleamine 2,3-dioxygenase 1 (IDO1)/ tryptophan 2,3-dioxygenase (TDO), further modulates fibrosis