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However, for the most accurate and reproducible scientific results, it should still be reconstituted strictly according to guidelines and stored at 28C after mixing

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The protein was dialysed against 10 mM TrisHCl pH 7.5, 10 mM MgSO 4 , 1 mM dithiothreitol and 0.1 mM ATP or ADP and concentrated to between 3 and 5 mg/ml prior to the crystallization