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For the reconstitution experiment, silent point mutations were introduced into the ABCC1 cDNA using PCR mutagenesis to avoid targeting by ABCC1 sgRNAs and the product was cloned into a pMSCV-PGK-BlastR retroviral expression vector using Gateway cloning

Seth Thompson Last Updated on May 28, 2026 Have you ever wondered why stress affects both your mind and body

Anderson P, Saltin B: Maximal perfusion of skeletal muscle in man

ISCs are involved in electron transport, substrate binding, enzymatic catalysis and play critical roles in maintaining genomic stability since several DNA polymerases and helicases require interaction with ISCs for proper functioning