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Tissue sections or cells were pre-incubated in 10% normal goat or donkey serum, 0.1% Triton-X 100 in PBS for 1 h and were incubated overnight at 4 C with the following antibodies: rabbit polyclonal anti-Sox2 (1:100, Cell Signaling Technology, #2748), chicken polyclonal anti-DCX (1:400, abcam, ab153668), cFos (1:2000, ABE457, Millipore)

1 The peptide enhances the proliferation and migration of endothelial cells and fibroblasts, which are essential for tissue repair

Inker LA, Eneanya ND, Coresh J, Tighiouart H, Wang D, Sang Y, et al

This characteristic is typical of hormetic substances and regulatory peptides, suggesting that DSIP works by triggering a cascade or signaling a threshold rather than by mass-action binding