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Nrf2/ARE, as one of the important mechanisms to protect the liver from oxidative stress, can play a protective role in liver inflammation, ischemia/reperfusion, fibrosis and regeneration by inducing target genes ( 4 is a strong hepatoxic and prooxidant agent widely used to induce hepatotoxicity, and its animal model has been widely used to screen the anti-hepatotoxicity and/or hepatoprotective activity of drugs ( 4 causes a decrease in liver cells, in the activities of antioxidant enzymes and increases the protein carbonyl content, which is a protein oxidation product, at the oxidative stress biomarker MDA level ( in vivo , different doses of midazolam pretreatment were given to mice with acute liver injury induced by CCl 4

It is generally assumed that needles of a shorter and smaller diameter provoke less painful insertions, bleeding, and bruising [7, 8]

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