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Incorporating in vivo analyses and biomarker assessments will enhance understanding of toxicant absorption and health effects

Carbonyls in e-cigarette aerosols were collected as follows: 1) the collector was rinsed with 5 ml acetonitrile, 2) the glass wool and beads were soaked with 1 ml DNPH solution [22 mM DNPH (Spectrum, New Brunswick, NJ) in acetonitrile with 25 mM hydrochloric acid (Sigma-Aldrich, MO, United States), pH3-4], then 3) 5-puffs of e-cigarette aerosol were generated while the collector was continuously wetted with the DNPH solution (0.5 ml/min rate)

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Ibuki Y, Toyooka T, Zhao X, Yoshida I