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Plant, Cell Environ 38:266279 Sheehan D, Meade G, Foley VM, Dowd CA (2001) Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily

Overexpression of human hepsin in adult mice with AAV administration For the APAP susceptibility test, mice were made to overexpress human hepsin in the liver through transduction with 2.5 10 10 viral genome (vg) per mouse of AAV-hHPN WT /hHPN RS /EGFP via retro-orbital injection

The soluble clarified fraction was recovered by centrifuging at 75,000 g for 20 min at 4 C and applied to a Superdex 200 26/600 column (Cytiva) for SEC, which was run as above