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When IB formation is a problem, recombinant protein synthesis should be carried out in the range 1525C, though one report described successful expression at 4C for 72 h (San-Miguel et al., 2013)

Other researchers have directly loaded therapeutic molecules into exosomes using drugs or gene tools, creating customized exosomes with enhanced neuroprotective, anti-inflammatory, or angiogenic properties, including GPX4 activators, clustered regularly interspaced short palindromic repeats (CRISPR)/Caspase-9, and specific miRNA overexpression methods (53, 55, 57, 58)

Zetterman T, Markkula R, Partanen JV, Miettinen T, Estlander AM,Kalso E

The sections were stained with hematoxylin and eosin (H&E) (Sakura finite), periodic acid-Schiff (PAS) (Scy Tek Laboratories, Logan, UT, USA) 23 , or MassonGoldner trichrome (MTC) (Sigma-Aldrich), and photographed using a BZ-9000 (Keyence, Osaka, Japan)