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Lang et al

If your results look slower or smaller than someone elses, its almost always one of these factors

Cells were stained on ice in dark for 15 min

A score of 1 was assigned if fluorescence was visible within the cytosol, while a score of 0 was assigned if fluorescence was present in the nucleus but the cytosolic signal failed to exceed that of the age-matched, non-transgenic N2 worms