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3a), likely weakening the interaction between the catalytic core and the pHAT domains, resulting in no observed density for the pHAT domains in the cryo-EM structure of Ta OGA (Fig

To 50 l in the wells of a 96-well plate (kept on ice), an equal volume of 2x GSH-Glo reagent containing GSH and the luciferin derivative was added with incubation for 30 min in the dark followed by 15 min with luciferin detection reagent (100 l)