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This actin-buffering capacity is critical for dynamic cellular processes including: Cell migration: Rapid actin polymerization at the leading edge Morphological changes: Cytoskeletal reorganization Intracellular transport: Actin filament dynamics The peptide contains a conserved LKKTETQ actin-binding motif that is essential for its interaction with G-actin

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Finally, the detected spots were averaged and a 20 pixel (400 nm) thick intensity line profile vertically along the peak of the Bassoon was measured