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To benchmark our approach against FI and established metabolomic clocks, we constructed comparator models that reflect key elements of prior frameworks trained on age 17 or untransformed metabolite levels 19 , though these models were developed on targeted NMR platforms and focused on age or mortality rather than health decline

Generally, a GSH content of 35 % could be obtained using strains selected via classical selection strategy, but the exact mechanisms underlying higher GSH accumulation in these mutants has remained rather obscure

The more marked activity of both enzymes on isothiocyanates is due to a better apparent affinity ( K m ) of the enzymes for substrates and a pretty good turnover ( k cat ) number of the enzyme for PITC and BITC

Ditzel et al