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Notably, the lack of schistocytes and a normal Coombs test support the conclusion that the anemia resulted from ineffective erythropoiesis rather than actual hemolysis

Subsequently, 100 l of BacTiter-Glo reagent was added to each well and the plate was shaken on an orbital shaker for 5 min before luminescence was measured

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In addition, it plays a role in the clotting cascadea sequence of reactions that convert clotting factors into enzymes and ultimately leads to the production of thrombin and fibrin