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DNA templates for in vitro transcription of sgRNA were generated by annealing a scaffold oligonucleotide sequence: AAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC, with gene-specific oligonucleotides containing the SP6 promoter sequence (GCGATTTAGGTGACACTATA), a 20-base target sequence (see Table S1), and a complementary sequence to the scaffold oligo (GTTTTAGAGCTAGAAATAG)

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Olive JK, Zhou N, Mitchell KG, Corsini EM, Hofstetter WL, Mehran RJ, Rice DC , Sepesi B, Swisher SG, Vaporciyan AA, Walsh GL, Antonoff MB