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The Enterobacteriaceae relative amounts were normalized with the 16S rRNA housekeeping gene amplified with the UniF (ACTCCTACGGGAGGCAGCAGT) and UniR (ATTACCGCGGCTGCTGGC) specific primer pair 38

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4c), an observation that is in agreement with our biochemical analyses (Table 1)

A separate line of investigation, work by Pollard and colleagues, demonstrated that GHK-Cu could reverse detrimental changes in irradiated cultured human fibroblasts: cells exposed to significant radiation and then treated with GHK-Cu at very low concentrations displayed a growth pattern and growth-factor secretion profile comparable to non-irradiated control cells, including increased secretion of bFGF and VEGF