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Thus, being able to measure all forms of GSH in one well significantly reduced the sample volume required for our experiment and mitigated inter-plate variation as a confound in data analysis

3.5 Region-specific spatiotemporal patterns of demyelination and remyelination At the CNS, demyelination was assessed by immunolabelling 30 m sagittal brain cryosections for MBP ( Figure 5A ) as well as by analyzing gene expression levels of myelin basic protein (MBP) and proteolipid protein (PLP) ( Figure 5B ) in whole brain