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2 Materials and methods 2.1 Protein structures and evaluation The hMRP1 ( and hMRP7 ( structures were predicted using AlphaFold ( Evaluation of the AlphaFold predicted model protein structures was performed using pLDDT (predicted Local Distance Difference Test) (Sippl, 1993), ERRAT (Williams et al., 2018)) integrated within the SWISS-MODEL workspace (Waterhouse et al., 2018)

Images were acquired using the Leica Application Suite AV (v 2.7.3.9723) software (Leica Microsystems)

Garcia-Rodriguez, Marco A

Indeed mutation of Thr 365 to alanine in UGT74F1 decreased SAG activity by 75% and increased SGE activity by 300%, suggesting an important role of this residue in catalysis and substrate recognition