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bEnd.3 cells from each treatment group were incubated at 37 C for 30 min in medium lacking FBS that contained 10 M DCHF-DA and then replaced with fresh medium with ionizable molecules at doses of 0.25 mg/mL (and incubated for 2 h) or 0.05 mg/mL (and incubated for 1 h, 4 h, 12 h or 24 h)

BH3-only proteins, upstream of the core Bcl-2 family proteins, can activate Bak and Bax, and we previously reported that BH3-only proteins, Bid and Bim, were involved in regulating hepatocyte apoptosis

76 p53 suppresses tumor through regulating its target gene, glutamine synthase 2 (GLS2), which exerts antioxidant defense function