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For studies with measurements of plasma insulin and glucagon concentrations, plasma was collected by tail vein bleeding at timepoints 0, 60, 120 and 240 min

All cells were synchronized to quiescence by being cultured in serum-free medium for 24 to 48 h and then treated with different stimuli as follows: (1) advanced glycation end products (AGEs) (0, 25, 50, or 100 g/mL in culture medium), with medium containing an equal concentration of BSA as a control

Notably, we observed decreased expression of REG3G in both jejunum and ileum, despite the expression of IL-22 only being reduced in jejunum