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A minor component of APAP is also oxidized by the microsomal cyto-chrome P450 system, predominantly by Cyp2E1 and Cyp1A2 [11], to form a reactive metabolite, N -acetyl- p -benzoquinone imine (NAPQI) [12]

A case in point, when 2 different mouse strains were exposed to therapeutic or hepatotoxic doses of APAP, depletion of GSH or APAP protein adduct formation was only detected in the liver but not in the whole brain or individual brain regions, suggesting that there is no evidence for the formation of the reactive metabolite NAPQI in the brain of adult mice [185]