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The cells were sub-cultured, when the cells are 80% confluent, using a cell scraper (AGC Techno Glass Co., Ltd) after processing by DPBS() (no calcium, no magnesium, Thermo Fisher Scientific Inc.) and CTK solution

PregS potentiates recombinant NMDARs with GluN1-1a/GluN2B through a steroid modulating domain in GluN2B that also modulates tonic proton inhibition and is pH independent

FDA staff and opposing groups argue that adding these compounds to the legal compounding list without adequate human safety data would legitimize unvetted foreign supply chains and create a false assurance of safety through the appearance of regulatory sanction

Cells were treated with vehicle (DMSO) or DMDD for 48 h after incubation with NG or HG DMEM medium for 24 h