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Further preclinical studies have demonstrated that acupuncture at Baihui (GV20), Yintang (EX-HN3), and other related acupoints significantly inhibits microglial polarization toward the pro-inflammatory M1 phenotype and reduces the expression of TNF- and IL-1 by suppressing the Toll-like receptor 4 (TLR4)/Myeloid differentiation primary response 88 (MyD88)/NF-B signaling cascade, thereby attenuating neuroinflammation (Jiang et al., 2024)

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