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doi: 10.18632/oncotarget.20165 183 SontheimerHBridgesRJ

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The reaction mixture, consisting of 1 g RNA template, 1 l iScript Reverse Transcriptase, 4 l 5 iScript Reaction Mix, and Nuclease-free water, was subjected to priming at 25 C, reverse transcription at 46 C, and inactivation at 95 C