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Then 5.0 L aliquot was treated with 8.7 L of sample buffer (50 mM Tris-HCl, 2.0% (v/v) SDS, 6.0% (v/v) 2-mercaptoethanol, 10% (v/v) glycerol and 0.050% (w/v) bromophenol blue in H 2 O) and heated at 100 C for 2 min