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The membrane was blocked with Blocking One (Nacalai Tesque, Inc., Kyoto, Japan) for 1 h and then incubated with a primary antibody overnight at 4C

To have a more general overview of the multiple functions of Dcr-2 in flies and mosquitoes, immunoprecipitation experiments were carried out and its protein interactome was determined by mass spectrometry (136, 139, 142)

Not by chance, COPD and CVD share common risk factors, pathophysiological processes, clinical signs, and symptoms [30]

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