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The length of the fifth exon of 13 GhGPXs was also similar, with a length of 180 bp in GhGPX6, GhGPX9 and GhGPX10 , and other GhGPXs were 168 bp

Typically, the method, such as that described by Chen et al., (PNAS 94:10756-61 (1997), uses a locus-specific oligonucleotide primer labeled on the 5 terminus with 5-carboxyfluorescein (F AM)