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Gln solved in aqueous solution constructs the cyclized compound pyroglutamic acid (Figure 1), which is an uncommon amino acid associated with metabolism problems [1]

Typically, we suggest the following: - Biotinylated oligonucleotides: 0.5 M -0.01 M diluted in 5 x SSCT buffer pH=7.0 - Biotinylated peptides: 1 g/mL - 1 ng/mL diluted in a PBST buffer pH=7.2 - Biotinylated proteins: 5 g/mL - 0.05 g/mL diluted in a PBST buffer pH=7.2 More information regarding Nunc Immobilizer Streptavidin Plates/Strips can be found on the Thermo Scientific Nunc Immobilizer Streptavidin Instruction Protocol (link below): Which types of Nunc Immobilizer Streptavidin Plates/Strips should I use for various detection methods

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Semin Fetal Neonatal Med 22(1):2329