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Since increased oxidative stress, as occurs in response to acidosis, is associated with metastasis and drug resistance, it seems logical for cells to reprogram their metabolism to cope with this type of increased stress

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The samples were then incubated overnight at 4 C with primary antibodies -III-tubulin (1:100, ab7751, clone TU-20, lot GR3238448-11, Abcam), -SMA (1:25, ab7817, clone 1A4, lot GR119216-7, Abcam), type III collagen (1:100, Acris AF5810, clone III-53, lot A130097BH) and CD45 (1:100, ab23, clone UCH-L1, lot GR3189280-2, Abcam) in 2.5% BSA