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doi: 10.1186/s12870-020-02655-2 57 WuW.ZhuS.XuL.ZhuL.WangD.LiuY.et al

in such a case, the protein will be translocated to the periplasm or the medium, though often at low concentrations, and the tagged protein can be readily purified from the culture medium after osmotically shocking the cells Some of currently used tags are summarized by Lichty et al (2005)