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Discovery of a bioactive inhibitor with a new scaffold for cystathionine gamma-lyase

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Immunoblotting Immunoblotting after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the cytosolic fractions to confirm the absence of any mitochondrial protein, was carried out using standard protocols, with antibodies specific for yeast cytosolic glucose-6-phosphate dehydrogenase (anti-G6PD, Sigma-Aldrich, A9521, 1:7500) and mitochondrial heat shock protein 60 (anti-Hsp60, Abcam, ab59458, 1:1000)

Alternatively, patients may present with peripheral sensory neuropathy as a result of cumulative dose