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Under real-time imaging guidance, precise needle sampling should be performed on regions with distinct characteristics to minimize errors caused by tumor heterogeneity [Citation194]

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For the thickness of the retinal tissue, internal plexiform layer, and internal nuclear layer, the measurements (ISSA program (VAMSTEC, Zagreb, Croatia) from the area of maximal tissue damage detected using semi-serial sections, five high-power fields were randomly selected for analysis, were made with an ocular micrometer at 20 magnification within 0.5 mm from the optic disc

Once activated, these cells and their cytokines circulate systemically, including to the brain, thereby linking gut immunity to CNS homeostasis