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New collection tubes were used after each step of centrifugation so that the exterior of the column never came in contact with previously discarded flowthrough

Blood 92(10 Suppl 1):246b, 1998

For experiments testing the impact of transposon insertions or gene deletions on biofilm formation, we used EcN clbQ :: kan (which lacks a gene in the colibactin biosynthesis pathway) as the WT reference strain to enable the use of kanamycin throughout the experiments