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The tight junction is composed of several transmembrane and cytosolic proteins, including occludin, claudins, zonula occludens (ZOs), tricellulin, cingulin, and junctional adhesion molecules (JAM), which interact with each other, as well as with the cytoskeleton, and form a complex architecture 27

While many people get enough B12 from their diet or oral supplements, some individuals may experience deficiency due to absorption problems, dietary restrictions, or certain medical conditions