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DAPI solution (5 g ml 1 , 0.2 ml) was added to stain the cells, which then remained at room temperature for 15 min, protected from light, and were then rinsed three times with PBS solution and blocked and recorded under a confocal microscope

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Notably, this nanoparticle effectively inhibits the 20 S proteasome without eliciting endocrine toxicity or cytotoxicity toward normal cellular functions

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