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SKU: 77269936844
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"The RalB-RLIP76 Complex Reveals a Novel Mode of Ral-Effector Interaction"

doi: 10.1007/s00232-004-0731-6

Reduced and alkylated samples were digested with Lysyl endopeptidase (LysC, Wako Chemicals USA) at 1:25 enzyme:protein overnight at room temperature, diluted to 1 M urea with 100 mM EPPS pH 8.1, and digested with trypsin (Promega) at a ratio of 1:15 enzyme:protein for 6 h at 37 C