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Involucra los centros de integracin hipotalmicos: El hipotlamo funciona como el regulador principal de los ritmos circadianos, la sealizacin endocrina y el equilibrio autnomo

Separation of soluble and insoluble components Single mouse hippocampal tissue was isolated, and 300 l mixed protein lysis solution with RIPA (Applygen, C1053), a protease inhibitor (Merck, 539134), and a phosphatase inhibitor (Merck, 524628) (=100:1:2) were added