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The LAAM GSH-CQDs exhibited a shorter tumor cell uptake time from time-dependent intracellular fluorescence experiments (4 and 6 h for LAAM GSH-CQDs and LAAM TC-CQDs, respectively) and shorter tumor accumulation time from in vivo fluorescence imaging experiments (5 and 8 h for LAAM GSH-CQDs and LAAM TC-CQDs, respectively)

Oldfield L, Costello E

Then 200 ul aliquot was taken into a small tube, sealed and kept at +4 C for additional time interval of 8 to 15 hours followed by transferring into 20 C freezer