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Similarly, treating the DRG neurons with paclitaxel significantly hampered microtubule dynamics, promoted the formation of retraction bulb-like structures at the distal growing tips (Gornstein and Schwarz, 2017), and thus markedly impaired the neurite outgrowth of cultured DRG neurons (Chine et al., 2019a)

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The cells were resuspended in binding buffer (20 mM Tris-HCl pH 7.0, 300 mM NaCl, 10 mM or 20 mM imidazole, 2 mM -mercaptoethanol) supplemented with 0.2 mM AEBSF-HCl

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