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GSH Quantification Glutathione and GSSG were measured in lysates from cells in 6-well plates as previously described ( ROS Quantification by Spin-Trapping and EPR Spectroscopy Cells (5 10 6 /0.1 ml) were stimulated for 2 h with LPS, then incubated with 50 mM 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO), synthetized as described previously ( Cell Viability Assay Cells were seeded in 96-well plates at 25,000/well in complete medium

Methylcobalamin injections are administered by licensed clinicians using medical grade protocols and are generally well tolerated

An Annexin V-EnzoGold (enhanced Cyanine-3) conjugate enables the detection of externalized phosphatidylserine (PS) (a marker of early apoptosis) distinct from fluorescein or GFP signals

The cell is protected from large shifts in redox equilibrium by active extracellular transport of GSSG or reaction with protein sulfhydryl groups and the formation of mixed disulfides