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Evidence that prolyl endopeptidase participates in the processing of brain angiotensin

A.SantosH

Through the inspection cell sensitivity of candidate MIM and apoptosis are reacted and to assess morphocytology/physiological variation.Like these experiments of carrying out of describing in detail among the embodiment 3.In brief, the candidate MIM with variable concentrations handles one group of cell line being made up of at least a control cells system and at least a cancerous cell line.Through assessing the sensitivity of cell line to potential MIM at monitoring cell survival rate on the different time and on the concentration in a series of application.Assess the apoptosis reaction of cell through for example using with the protein reagent that is connected of flow cytometry method combination to potential MIM.Connect protein reagent and comprise the combination of two kinds of dyestuff 7AAD and annexin-V-PE, and allow quantitatively to be in early days and late period apoptotic cell colony.For example ApostrandTM ELISA method capable of using is used the other apoptosis algoscopy of measuring single stranded DNA.Assessment and sensitivity and apoptosis reaction that relatively diseased cells is and control cells is.Induce diseased cells to be accredited as MIM with respect to the molecule of Normocellular apoptosis reaction with showing difference cytotoxicity and/or difference

Stimulation by alpha-adrenergic mechanisms of the secretion of growth hormone-releasing factor (GRF) from perifused rat hypothalamus