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Thereafter, the absorbance measured at 412 nm was read against a blank containing 3.5 ml of buffer using spectrophotometer and the equivalent GSH content was estimated from the standard GSH curve provided in the kit

Springer, Dordrecht, The Netherlands, pp

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The glutaminase I system, which is now known as the glutaminase or phosphate-activated glutaminase system, comprises kidney (GLS or KGA) and liver (GLS2 or LGA) isozymes activated by phosphate (111), while the glutaminase II system is activated by -keto acids (112)