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Hyperinsulinemia is linked to various cancers, including PC

For mesenteric lymph nodes, spleen, and thymus, tissues were ground in a 6-well plate through a 70m cell strainer (Corning 352350) in T Cell Media (TCM) consisting of RPMI 1640 (Gibco 21870) supplemented with 10% FBS (Gibco 10437), 1% Penicillin-Streptomycin (Gibco 15140), 1% L-glutamine (Gibco 25030), 1% sodium pyruvate (Gibco 113690), 1% Non-Essential amino acids (Gibco 11140), 1% HEPES (Gibco 15630), and 50M -mercaptoethanol (Sigma M6250)

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