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Metabolomic analysis 4T1 cells were seeded in cell culture flasks (2 10 6 cells/flask) and incubated for 24 h

AMAP also undergoes extensive CYP-mediated bioactivation to chemically reactive species in vitro and in vivo (Rashed and Nelson 1989

For remaining experiments adult animals at an age of 1012 weeks were used

While the experimental models employed in PD have shown connections to human health, it remains a daunting task to translate mechanisms of action observed in in vitro and in vivo studies of natural compounds to clinical outcomes in human trials, where it is a critical step in drug development