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A major disadvantage for using GST as solubility and affinity tag relies on its oligomerized form: GST has four solvent exposed cysteines that can provide a significant oxidative aggregation (Kaplan et al., 1997), making it a poor choice for tagging oligomeric target proteins (Malhotra, 2009)

Note from this diagram how the nonoxidative phase makes several connections with glycolysis through formation of glyceraldehyde-3P, a 3C intermediate), and fructose-6-P, a 6C intermediate

Effect of sonic versus manual supervised toothbrushing on both clinical and biochemical profiles of patients with desquamative gingivitis associated with oral lichen planus: a randomized controlled trial